Synthesis of hybrid materials, immobilization of lipase in SBA-15 modified with CaO

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J. C. Méndez
U. Arellano
S. Solís
M. Asomoza
V. H. Lara
A. J. Padilla
J.A. Wang

Abstract

Mesoporous silica materials (SBA-15) doped with calcium (Ca) at 1, 3 and 5% w/w were synthesized in order to immobilize candida rugosa lipase (CRL), through the adsorption method. It was found that calcium (Ca) is present as CaO, highly dispersed on the surface of SBA-15, the specific area of SBA-15 decreases on incrementing CaO content. The environmental pH influences the adsorption of CRL due to the polarization of positive charges below the isoelectric point (IEP) whereas the Ca-SBA-15 materials present negative charges. The adsorption model proposed for CRL is about the accessible surface of the material, as this has a size of 7 nm and the Ca-SBA-15 present pores at an interval of 3 nm.  

 

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How to Cite
Méndez, J. C., Arellano, U., Solís, S., Asomoza, M., Lara, V. H., Padilla, A. J., & Wang, J. (2019). Synthesis of hybrid materials, immobilization of lipase in SBA-15 modified with CaO. Journal of Applied Research and Technology, 16(6). https://doi.org/10.22201/icat.16656423.2018.16.6.747
Author Biographies

J. C. Méndez

Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186, Iztapalapa, México D.F., México.

U. Arellano

Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186, Iztapalapa, México D.F., México.

S. Solís

Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186, Iztapalapa, México D.F., México

M. Asomoza

Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186, Iztapalapa, México D.F., México

V. H. Lara

Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186, Iztapalapa, México D.F., México.

A. J. Padilla

Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186, Iztapalapa, México D.F., México.

J.A. Wang

ESIQIE, Instituto Politécnico Nacional, Av. Instituto Politécnico Nacional, s/n, Col. Zacatenco, 07738, Mexico City, Mexico