Modeling of microbial growth and ammonia consumption at differenttemperatures in the production of a polyhydroxyalkanoate (PHA) biopolymer

Abstract

2015AbstractModeling of microbial growth and ammonia consumption at different temperatures was developed in the production of a polyhydroxyalkanoate(PHA) biopolymer in Pseudomona fluorescens in the range of 25–35?C. A logistic model was employed to predict accurately the microbial growthlimiting conditions of nitrogen. A new model based in a mixed mathematical equation comprising a logistic model, and a magnetic saturation modelresulted appropriate to estimate the ammonia consumption under limiting conditions. Favorable conditions for PHA production in P. fluorescenswere found at temperature of 30?C, reaching the maximum biomass concentration of 2.83 g L?1, and consuming 99.9% of the initial ammonia, toproduce 2.13 g L?1of PHA. The proposed models could be useful to predict the behavior of a fermentation process to produce PHA in real time.All Rights Reserved © 2015 Universidad Nacional Autónoma de México, Centro de Ciencias Aplicadas y Desarrollo Tecnológico. This is anopen access item distributed under the Creative Commons CC License BY-NC-ND 4.0.