Heating ability and hemolysis test of magnetite nanoparticles obtained by a simple co-precipitation method

Abstract

The present paper reports the heating ability and hemolysis test of magnetite nanoparticles (MNPs) for biomedical applications, obtained bya novel and easy co-precipitation method, in which it is not necessary the use of controlled atmospheres and high stirring rates. Different molarproportions of FeCl₂:FeCl₃ (2:1 and 3:2 respectively) were used and the obtained MNPs were analyzed by X-ray diffraction, vibrating samplemagnetometry and transmission electron microscopy. The heating ability was evaluated under a magnetic field using a solid state induction heatingequipment at two different frequencies (362 and 200 kHz). Additionally, a hemolysis test was performed according to the ASTM method. Theobtained ferrites showed a particle size in the range of 8–12 nm and superparamagnetic behavior. The MNPs increased the temperature up to43.1?C in 5 min under a low magnetic field and showed non hemolytic effect up to 3 mg/ml. The MNPs obtained are highly potential materials forhyperthermia cancer treatment.